The course will cover two major areas: 1) the fundamental of molecular biology; 2) the application of molecular biology to engineering. The details are:1. History of molecular biology and relevance to environmental engineering.2. Introduction of molecular biology, cell structure, and genomic information. 3. Microbial performance — protein formation4. The correlation of gene sequence and microbial species. 5. Gene transfer, mutation, genetic recombination, and cloning. 6. Methods in microorganism observation and identification (FISH, DGGE-PCR, RFLP, microarray)7. Microbial community function8. Quantitative and qualitative molecular biology9. Applications in water and wastewater treatment10. Applications in soil system